| 產品名稱 | CMT-93 |
|---|---|
| 商品貨號 | B161441 |
| Organism | Mus musculus, mouse |
| Tissue | rectum |
| Product Format | frozen |
| Morphology | epithelial |
| Culture Properties | adherent |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease | polyploid carcinoma |
| Age | 19 months |
| Gender | male |
| Strain | C57BL/icrf |
| Storage Conditions | liquid nitrogen vapor phase |
| Images |  |
| Clinical Data | male |
| Antigen Expression | H-2b |
| Tumorigenic | Yes |
| Effects | Yes, in nude mice (Tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 10(7) cells) |
| Comments | Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium | The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing | Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended Medium Renewal: 2 to 3 times per week
|
| Cryopreservation | culture medium 95%; DMSO, 5% |
| Culture Conditions | Temperature: 37°C |
| Name of Depositor | LM Franks |
| Deposited As | Mus musculus |
| References | Franks LM, Hemmings VJ. A cell line from an induced carcinoma of mouse rectum. J. Pathol. 124: 35-38, 1978. PubMed: 722371 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
