| 產(chǎn)品名稱(chēng) |
pSW 11-1 [pSW11-1] |
| 商品貨號(hào) |
B161877 |
| Designations |
pSW 11-1 [pSW11-1] |
| GenBank Number |
L00045
|
| Species |
Homo sapiens, human |
| Depositors |
RA Weinberg |
| Applications |
NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone. |
| Vector |
Construct size (kb): 3.900000095367432 |
| Insert |
DNA: cDNA Insert lengths(kb): 1.100000023841858 Tissue: SW480 cell line DNA transferred into NIH 3T3 cells Gene product: Kirsten rat sarcoma 2 viral (v-Ki-ras2) oncogene homolog( c-Kirsten-ras proto-oncogene, oncogene KRAS2, cellular c-Ki-ras2 proto-oncogene) [KRAS2] |
| Insert Size (kb) |
1.100 |
| Media |
ATCC® Medium 1227: LB Medium (ATCC medium 1065) with 50 mcg/ml ampicillin
|
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Shipping Information |
Distributed: freeze-dried |
| Comments |
Restriction digests of the clone give the following sizes (kb): PstI--3.8; EcoRI--3.1, 0.6; PstI/EcoRI--2.6, 0.6, 0.5. Detects a TaqI polymorphism mapping to chromosome 6. A full-length Ki-ras cDNA capable of transformation if given a promoter. The PstI site on the 3' side of the insert appears to be missing. The insert contains HinfI (3), TaqI (3), Sau96I (2) and EcoRI sites. NIH 3T3 cells were transformed with DNA from the colon adenocarcinoma SW480 cell line to produce the cell line, SW2-3. DNA from SW2-3 was used to isolate the clone. |
| References |
Siracusa LD, et al. A molecular genetic linkage map of mouse chromosome 2. Genomics 6: 491-504, 1990. PubMed: 1970329
Threadgill DW, et al. Syntenic conservation between humans and cattle: II. human chromosome 12. Genomics 8: 29-34, 1990. PubMed: 2081597
McCoy MS, et al. Human colon carcinoma Ki-ras2 oncogene and its corresponding proto-oncogene. Mol. Cell. Biol. 4: 1577-1582, 1984. PubMed: 6092920
Barbara Weiffenbach, personal communication
|
