Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Product Format
frozen 1 mL per vial
Storage Conditions
-70°C or colder
Comments
Grows poorly in Balb/c mice.
Effect on Host
CPE, syncytia, rounding, sloughing
Recommended Host
Vero (ATCC® CCL-81™)
Growth Conditions
Temperature: 37°C
Recommendations For Infection: Plate cells 24-48 hours prior to infection and infect when cultures are 80-90% confluent. Remove medium and inoculate with a small volume of virus (e.g. 1 mL per 25 cm2) diluted to provide an optimal MOI (e.g. 0.1). Adsorb 1-2 hours at 37°C in a humidified 5% CO2 atmosphere, rocking every 20-30 minutes to redistribute inoculum. End adsorption by adding virus growth medium.
Incubation: 3-9 days
Name of Depositor
JE Crowe, National Institutes of Allergy and Infectious Diseases
Source
Secretion from endotracheal tube of human infant with pneumonia
References
Crowe JE, et al. Live subgroup B respiratory syncytial virus vaccines that are attenuated, genetically stable, and immunogenic in rodents and nonhuman primates.. J. Infect. Dis. 173: 829-839, 1996. PubMed: 8603960
