| 產品名稱 |
162-21.2 |
| 商品貨號 |
B163592 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Choriocarcinoma |
| Applications |
The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas. The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors. No staining was detected on peripheral blood cells. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Trop-1 antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Trop-1 antigen |
| Comments |
Animals were immunized with the human choriocarcinoma cell line BeWo. Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with an antigen (Trop-1) present on trophoblast cells and on human choriocarcinomas. The antigen has also been detected on 4 of 4 human choriocarcinoma cell lines tested, on a fibrosarcoma cell line (HT1080C), on an erythroleukemia cell line (K562) and on the majority of human malignant epithelial cells and fetal epithelial precursors. The gene encoding the trop-1 antigen has been cloned. No staining was detected on peripheral blood cells. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
LA Herzenberg |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lipinski M, et al. Human trophoblast cell-surface antigens defined by monoclonal antibodies. Proc. Natl. Acad. Sci. USA 78: 5147-5150, 1981. PubMed: 7029529
Strnad J, et al. Molecular cloning and characterization of a human adenocarcinoma/epithelial cell surface antigen complementary DNA. Cancer Res. 49: 314-317, 1989. PubMed: 2463074
Klein CE, et al. Expression of 38-kD cell-surface glycoprotein in transformed human keratinocyte cell lines, basal cell carcinomas, and epithelial germs. J. Invest. Dermatol. 95: 74-82, 1990. PubMed: 2366004
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