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• O.1 mM Non-Essential Amino Acids (NEAA)
• fetal bovine serum to a final concentration of 10%

Temperature: 37°C
Atmosphere: 5% CO₂

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Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,772,998 dated Jun 30 1998

Dasch JR, et al. Monoclonal antibodies to transforming growth factor-beta and methods of use. US Patent 5,783,185 dated Jul 21 1998

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.