| 產品名稱 | 1G3 |
|---|---|
| 商品貨號 | B163624 |
| Organism | Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type | hybridoma: B lymphocyte |
| Product Format | frozen |
| Morphology | lymphoblast |
| Culture Properties | mixed: adherent and suspension |
| Biosafety Level | 1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications | This antibody is used in studies of the MHC class I heavy chain FcRn heterodimers and their interaction with IgG. Ref   Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418 |
| Storage Conditions | liquid nitrogen vapor phase |
| Derivation | The cell line was established by Louis Gastinel and Pamela Bjorkman in 1992. Animals were immunized with and twice boosted with soluble neonatal Fc receptor (FcRn) in an adjuvant. Three days before fusion one mouse was boosted with purified FcRn. Spleen cells were fused with HL-1 Friendly myeloma cells (a derivative of P3X63Ag8.653 that is deficient in both hypoxanthine phosphoribosyl transferase [HPRT] and adenine phosphoribosyl transferase [APRT]). |
| Genes Expressed | immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers |
| Cellular Products | immunoglobulin; monoclonal antibody; against FcRn heavy chain heterodimers |
| Comments |
The antibody recognizes FcRn heavy chain heterodimers. FcRn is a heterodimer composed of a membrane bound heavy chain attached noncovalently to beta 2-microgloublin. It is structurally similar to class I major histocompatibility (MHC) molecules. The IG3 (ATCC CRL-2434) antibody recognizes two lipid linked forms of FcRn. These forms are truncated FcRn heavy chain paired with beta 2-microgloublin-DAF (FcRn beta-2 microglobulin DAF) and lipid-linked FcRn paired with unaltered beta 2-microgloublin (FcRn-DAF/beta 2-microgloublin). It also recognizes the FcRn-DAF construct. The 2G3 (ATCC CRL-2435) antibody fails to recognize the FcRn heterodimer form in which the heavy chain is attached to the lipid anchor (FcRn-DAF/beta 2-microgloublin) and it also fails to recognize the FcRn-DAF construct. Unlike 4C9 (ATCC CRL-2437), the monoclonal antibodies IG3 (ATCC CRL-2434) and 2G3 (ATCC CRL-2435) do not recognize rat beta 2-microglobulin alone or complexed with FcRn heavy chains. |
| Complete Growth Medium | HL-1 medium supplemented with 4 mM L-glutamine, 1 mM sodium pyruvate and 1% fetal bovine serum. HL-1 medium can be obtained from from Lonza (catalog number 77201).
|
| Subculturing | Subcultures are prepared by scraping. Dislodge cells from the flask substrate with a cell scraper. Aspirate gently and dispense into new flasks. Seed flasks at 2 x 105 viable cells/mL. Subcultivation Ratio: A subcultivation ratio of 1:5 every 3 to 4 day is recommended |
| Cryopreservation | HL-1 Complete Serum-free Medium, 75%; fetal bovine serum, 20%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.
|
| Culture Conditions | Temperature: 37°C |
| Isotype | IgG1 |
| Name of Depositor | PJ Bjorkman |
| Deposited As | mouse (B cell); mouse (myeloma) |
| Year of Origin | 1992 |
| References | Raghavan M, et al. Investigation of the interaction between the class I MHC-related Fc receptor and its immunoglobulin G ligand. Immunity 1: 303-315, 1994. PubMed: 7889418 |
| 梅經理 | 17280875617 | 1438578920 |
| 胡經理 | 13345964880 | 2438244627 |
| 周經理 | 17757487661 | 1296385441 |
| 于經理 | 18067160830 | 2088210172 |
| 沈經理 | 19548299266 | 2662369050 |
| 李經理 | 13626845108 | 972239479 |
