| 產品名稱 |
2.28 M1 [A2,28M1] |
| 商品貨號 |
B163629 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4). Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic |
| Cellular Products |
immunoglobulin; monoclonal antibody; against HLA A2, A28; cytotoxic |
| Comments |
Animals were immunized with human peripheral blood leukocytes (HLA phenotype A2,Bw6,Bw48,Bw60,Cw4). Spleen and lymph node cells were fused with Sp2/08A2 myeloma cells. The antibody is cytotoxic for a subpopulation of HLA A2 positive cells and HLA A28 positive cells at an appropriate titer. The antibody recognizes an epitope at the amino terminal end of the alpha 2 domain of the HLA-A2 molecule. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
- 0.05 mM 2-Mercaptoethanol
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 5 X 10 exp5 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgM |
| Name of Depositor |
F Grumet |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Hogan KT, Brown SL. Localization and characterization of serologic epitopes on HLA-A2. Hum. Immunol. 33: 185-192, 1992. PubMed: 1377666
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