亚洲.无码.制服.日韩.中I人妻无码久久精品人妻成人I制片厂91I国产真实91东北熟妇HDXXXI五月AVI四虎精品性爱I国产亚州avI日产精品久久久久I人人爱夜夜操

熱門搜索:A549    293T 金黃色葡萄球菌 大腸桿菌 AKK菌
購物車 1 種商品 - 共0元
當前位置: 首頁 > ATCC代理 > 20C2 [4H12:20C2.G6.C9]
最近瀏覽歷史
聯系我們
  • 0574-87157013
  • mingzhoubio@163.com
  • 浙江省寧波市鎮海區莊市街道興莊路9號
  • 創e慧谷42號樓B幢401室
20C2 [4H12:20C2.G6.C9]
20C2 [4H12:20C2.G6.C9]
規格:
貨期:
編號:B163639
品牌:Mingzhoubio

標準菌株
定量菌液
DNA
RNA

規格:
凍干粉
斜面
甘油
平板


產品名稱 20C2 [4H12:20C2.G6.C9]
商品貨號 B163639
Organism Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma)
Cell Type hybridoma: B lymphocyte
Product Format frozen
Morphology lymphoblast
Culture Properties suspension
Biosafety Level 1

Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.

Applications
The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution.
The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12.
The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12.
The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12.
Storage Conditions liquid nitrogen vapor phase
Derivation
The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution.
The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75.
Genes Expressed
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75
Cellular Products
immunoglobulin; monoclonal antibody; against human interleukin 12 (IL-12) p75
Comments
The hybridoma cell line 20C2 [4H12:20C2.G6.C9} was established by Richard Chizzonite in 1991 and subcloned three times by limiting dilution.
The hybridoma produces an antibody (IgG1) that binds the p75 heterodimer of human IL-12.
The antibody may be used in neutralizing and receptor binding assays; it will immunoprecipitate IL-12.
The antibody can be used as a capture antibody in sandwich EIA to detect the p75 form of human IL-12.
The 20C2 antibody does not bind the p40 or p35 subunits of IL-12.
The 20C2 cell line was formed by the fusion of NSO mouse myeloma cells with splenocytes from a Lewis rat inoculated with partially purified human IL-12 p75.
Complete Growth Medium Iscove's Modified Dulbecco's Medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.1 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%.
Subculturing Cultures can be maintained by addition of fresh Medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 x 105 viable cells/mL.  Maintain cultures at a cell concentration between 1 x 105 and 1 x 106 cells/mL. Maintain cell density between 1 x 105 and 1 x 106 viable cells/mL.

Medium Renewal: Add fresh medium every 2 to 3 days (as cell density increases)

Cryopreservation

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Culture Conditions
Temperature: 37°C
Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%
Isotype IgG1
Name of Depositor DH Presky
Deposited As rat (B cell); mouse (myeloma)
Year of Origin 1991
References

Chua AO, et al. Expression cloning of a human IL-12 receptor component--a new member of the cytokine receptor superfamily with strong homology to gp130. J. Immunol. 153: 128-136, 1994. PubMed: 7911493

Chizzonite R, et al. IL-12: monoclonal antibodies specific for the 40-kDa subunit block receptor binding and biologic activity on activated human lymphoblasts. J. Immunol. 147: 1548-1556, 1991. PubMed: 1715362

Chizzonite R, et al. IL-12 receptor. I. Characterization of the receptor on phytohemagglutinin-activated human lymphoblasts. J. Immunol. 148: 3117-3124, 1992. PubMed: 1578138

Desai BB, et al. IL-12 receptor. II. Distribution and regulation of receptor expression. J. Immunol. 148: 3125-3132, 1992. PubMed: 1578139

Gately MK, et alMeasurement of Human and Mouse Interleukin-12In: Gately MK, et alCurrent Protocols in ImmunologyNew York, N.Y.John Wiley and Sons1995.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.

Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.

梅經理 17280875617 1438578920
胡經理 13345964880 2438244627
周經理 17757487661 1296385441
于經理 18067160830 2088210172
沈經理 19548299266 2662369050
李經理 13626845108 972239479
主站蜘蛛池模板: 人人爱人人射 | 国产特级毛片aaaaaa毛片 | 久久久久中文 | 337p西西人体大胆瓣开下部 | 中文字幕在线视频精品 | 成人午夜精品久久久久久久3d | 中文欧美字幕免费 | 超碰在线人人 | 人人爱在线视频 | 免费观看一级视频 | 日日夜夜狠狠 | 麻花传媒mv免费观看 | 亚洲aⅴ一区二区三区 | 国产xx在线 | 色婷婷免费视频 | 国产涩涩在线观看 | 国产片网站 | 国产又黄又猛又粗 | 国产不卡在线 | 日韩av高清在线观看 | 五月婷婷黄色网 | 久久国内视频 | 国产精品短视频 | 91热精品 | 中文字幕av全部资源www中文字幕在线观看 | 日韩欧美观看 | 亚洲精品午夜久久久久久久久久久 | 91x色| 欧美日韩中文字幕在线视频 | 久久午夜色播影院免费高清 | 丝袜美腿在线视频 | 18国产精品白浆在线观看免费 | 波多野结衣在线观看一区 | 久久综合福利 | 亚洲资源 | 久久激情综合 | 亚洲另类人人澡 | 69人人| 亚洲综合色婷婷 | 激情五月婷婷综合网 | 最近免费中文字幕 | 99精品欧美一区二区三区 | 久久久国产一区二区三区四区小说 | 国产成人精品av在线观 | 久久久久9999亚洲精品 | 婷婷黄色片| 久久久电影网站 | 久久综合久久综合久久综合 | 99久免费精品视频在线观看 | 91视频在线免费看 | 欧美a级片免费看 | 精品久久久免费视频 | 看毛片的网址 | 国产精品成人久久久 | 激情久久伊人 | 精品字幕在线 | 99精品在线观看视频 | 精品一区二区三区久久久 | 久久久久国产一区二区三区四区 | 国产又粗又猛又黄视频 | 国产精品一区二区久久精品 | 91福利影院在线观看 | 久久久久久久国产精品 | 美女福利视频网 | www成人精品 | 欧美日韩激情网 | 午夜av剧场 | 国产免费av一区二区三区 | 国产视频综合在线 | 在线视频91 | 中文字幕免费成人 | 超碰97人人干 | 91探花系列在线播放 | 婷婷色5月 | 欧美一级淫片videoshd | 美女视频a美女大全免费下载蜜臀 | 国语精品视频 | 久久久精品 一区二区三区 国产99视频在线观看 | 免费观看久久 | 日韩高清在线一区二区三区 | 91亚州| 999国产在线 | 91精品成人久久 | 综合激情| 九九免费观看全部免费视频 | 最近日本韩国中文字幕 | 欧美91精品| 久久伊99综合婷婷久久伊 | 国产日韩欧美在线 | 91亚洲国产成人久久精品网站 | 国产一区二区久久 | 日日夜夜天天综合 | 亚洲免费观看视频 | 91精品国产欧美一区二区 | 免费福利在线观看 | 911精品视频 | 四虎国产永久在线精品 | 国产午夜精品免费一区二区三区视频 | 国产精品小视频网站 |