| 產品名稱 |
240-13D10 |
| 商品貨號 |
B163647 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene). Weaker activity against src and fgr was also detected. In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines. |
| Derivation |
Spleen cells were fused with Sp2/0 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a c-yes synthetic oncogene peptide |
| Comments |
Animals were immunized with a synthetic polypeptide with the sequence LMKLCWKKDPDERPTC corresponding to peptides 502 to 517 of the c-yes oncogene (formerly 770 to 793 of the v-yes oncogene). Spleen cells were fused with Sp2/0 myeloma cells. In ELISA tests, the antibody reacts strongly with synthetic peptides representing the yes, abl, and ros oncogenes. Weaker activity against src and fgr was also detected. In immunoblot assays, a protein of approximately 70 kd is detected in a wide variety of cell lines. The IgG1 reactivity of the antibody with the v-yes peptide (778 to 793) was confirmed by ELISA. IgG1 was strongly positive while IgG2b was only slightly positive. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density) Maintain cell density between 2 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 X 10 exp5 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 and IgG2b |
| Name of Depositor |
National Cancer Institute |
| Deposited As |
mouse (B cell); mouse (myeloma) |
