| 產品名稱 |
2D10 |
| 商品貨號 |
B163676 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Tissue |
lymph node, popliteal |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Lymphoma |
| Age |
adult |
| Strain |
Lewis |
| Applications |
The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen. |
| Derivation |
Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against mouse B7.2 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse B7.2 |
| Comments |
Animals were immunized in the hind footpad with irradiated 5C2 mouse B cell lymphoma cells that had been cultured in the presence of dibutyryl-cAMP. Popliteal lymph node cells were fused with Sp2/0-Ag14 myeloma cells. The antibody will neutralize the B7.2 antigen, but does not react with the B7.1 antigen. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium:
- 0.05 mM 2-mercaptoethanol
- fetal bovine serum to a final concentration of 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 0.5 X 10 exp5 to 1 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b |
| Name of Depositor |
SE Buchholz |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Powers GD, et al. Expression and functional analysis of murine B7 delineated by a novel monoclonal antibody. Cell. Immunol. 153: 298-311, 1994. PubMed: 7509723
Nabavi N, et al. Signalling through the MHC class II cytoplasmic domain is required for antigen presentation and induces B7 expression. Nature 360: 266-268, 1992. PubMed: 1279442
Chen C, et al. Monoclonal antibody 2D10 recognizes a novel T cell costimulatory molecule on activated murine B lymphocytes. J. Immunol. 152: 2105-2114, 1994. PubMed: 7510738
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