| Comments |
Mice were immunized with the 17D strain of yellow fever virus. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody does not cross-react with other flaviviruses. Antibody reactivity can be assayed by hemagglutination inhibition, complement fixation and immunofluorescence. Tested and found negative for ectromelia virus (mousepox). |
| References |
Schlesinger JJ, et al. Monoclonal antibodies distinguish between wild and vaccine strains of yellow fever virus by neutralization, hemagglutination inhibition and immune precipitation of the virus envelope protein. Virology 125: 8-17, 1983. PubMed: 6187129
Monath TP, et al. Yellow fever monoclonal antibodies: type-specific and cross-reactive determinants identified by immunofluorescence. Am. J. Trop. Med. Hyg. 33: 695-698, 1984. PubMed: 6206738
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
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