| 產品名稱 |
36F-18C [36F] |
| 商品貨號 |
B163718 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against sheep (ovine) CD2 |
| Cellular Products |
immunoglobulin; monoclonal antibody; against sheep (ovine) CD2 |
| Comments |
Animals were immunized with phytohemagglutinin-stimulated sheep T cells. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody is specific for sheep CD2 and precipitates a 50000 to 55000 dalton molecule from lysates of sheep lymphocytes. It does not inhibit cytotoxic T lymphocyte mediated killing or mixed lymphocyte reactivity. |
| Complete Growth Medium |
CULTURE MEDIUM: Iscove's modified Dulbecco's medium, 90%; fetal bovine serum,
10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2a |
| Name of Depositor |
CR Mackay |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Mackay CR, et al. Unusual expression of CD2 in sheep: implications for T cell interactions. Eur. J. Immunol. 18: 1681-1688, 1988. PubMed: 2462499
|
