| 產(chǎn)品名稱 |
38-1 |
| 商品貨號 |
B163721 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with a poorly conserved epitope on CB4 and neutralizes a restricted set of human CB4 isolates. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing |
| Cellular Products |
immunoglobulin; monoclonal antibody; against coxsackievirus B4 (CB4); neutralizing |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with coxsackievirus B4 (JVB strain, ATCC VR 184). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with a poorly conserved epitope on CB4 and neutralizes a restricted set of human CB4 isolates. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Culture Medium: Dulbecco's modified Eagle's medium (containing 1 mM sodium
pyruvate) with 4.5 g/L glucose, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
BS Prabhakar |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Prabhaker BS, et al. High frequency of antigenic variants among naturally occurring human Coxsackie B4 virus isolates identified by monoclonal antibodies. Nature 300: 374-376, 1982. PubMed: 6183593
Prabhaker BS, et al. Detection of conserved and nonconserved epitopes on Coxsackievirus B4: frequency of antigenic change. Virology 146: 302-306, 1985. PubMed: 2413617
Webb SR, et al. Neutralization epitope diversity of Coxsackievirus B4 isolates detected by monoclonal antibodies. J. Med. Virol. 20: 9-15, 1986. PubMed: 2428930
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