| 產(chǎn)品名稱(chēng) |
3B18 |
| 商品貨號(hào) |
B163729 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
mixed: adherent and suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Carcinoma |
| Strain |
Strain: BALB/c (B cell); BALB/c (myeloma) |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with PAI myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against a human mammary tumor cell cytoplasmic antigen |
| Cellular Products |
immunoglobulin; monoclonal antibody; against a human mammary tumor cell cytoplasmic antigen |
| Comments |
Animals were immunized with MCF7 cells (ATCC HTB-22). Spleen cells were fused with PAI myeloma cells. The antibody cross-reacted with some cases of fibrocystic mammary disease and one of two colon carcinomas tested. It did not react with normal tissue nor with 17 of 18 non-mammary malignancies tested. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 0.1 mM hypoxanthine, 0.02 mM thymidine, 0.01 mM 2-mercaptoethanol, and non-essential amino acids, 90%; horse serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by transferring floating cells to additional flasks. Attached cells may be subcultured by scraping. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1 |
| Name of Depositor |
Salk Institute for Biological Studies |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Dulbecco R, et al. Monoclonal antibody specific for a mammary tumor cytoplasmic antigen. US Patent 4,628,032 dated Dec 9 1986
|
