| 產品名稱 |
44aacb |
| 商品貨號 |
B163749 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Applications |
The antibody reacts with human CD11b, and blocks a variety of CD11b/CD18 mediated activities including neutrophil - substrate adherence and C3bi rosette formation on neutrophils, monocytes, macrophages and some NK cells. The antibody cross-reacts with CD11b from non-human primates. |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD11b |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD11b |
| Comments |
Animals were immunized with human acute monocytic leukemia cells (FAM M6). Spleen cells were fused with NS-1 myeloma cells. The antibody reacts with human CD11b, and blocks a variety of CD11b/CD18 mediated activities including neutrophil - substrate adherence and C3bi rosette formation on neutrophils, monocytes, macrophages and some NK cells. The antibody cross-reacts with CD11b from non-human primates. |
| Complete Growth Medium |
The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium: - heat-inactivated gamma globulin free horse serum to a final concentration of 10%
1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
RF Todd |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Arnaout MA, et al. Inhibition of phagocytosis of complement C3- or immunoglobulin G-coated particles and of C3bi binding by monoclonal antibodies to a monocyte- granulocyte membrane glycoprotein (Mol). J. Clin. Invest. 72: 171-179, 1983. PubMed: 6874946
Simon RH, et al. Neutrophil-induced injury of rat pulmonary alveolar epithelial cells. J. Clin. Invest. 78: 1375-1386, 1986. PubMed: 3771800
Ismail G, et al. Prevention of pulmonary injury in isolated perfused rat lungs by activated human neutrophils preincubated with anti-Mo1 monoclonal antibody. Blood 69: 1167-1174, 1987. PubMed: 3030466
Dana N, et al. Two functional domains in the phagocyte membrane glycoprotein Mo1 identified with monoclonal antibodies. J. Immunol. 137: 3259-3263, 1986. PubMed: 2430017
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