| 產(chǎn)品名稱 |
5637 |
| 商品貨號(hào) |
B163788 |
| Organism |
Homo sapiens, human |
| Tissue |
urinary bladder |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade II carcinoma |
| Age |
68 years adult |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
modal chromosome number = 67; range = 59 to 71The stemline modal chromosome number is 67 occurring at 36%, and polyploidy at 0.6%. Fourteen marker chromosomes were common to these cells: 3q+, 11q+, i(13q), t(9q21q), i(17q), i(21q) and 8 others of unknown origin. Additional markers: der(5)t(5;7)(q31;p11) and 1p were found that were specific to a minor subpopulation. Microchromosomes (larger than double minutes) and double minutes (DM) were also found in a small population of cells. Normal N13 was absent; and N21 was found only in the minor subpopulation.One, or occasionally two, Y chromosomes were present in each cell. |
| Clinical Data |
68 years adult Caucasian male |
| Tumorigenic |
Yes |
| Effects |
Yes, within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X,Y CSF1PO: 11 D13S317: 11 D16S539: 9 D5S818: 11,12 D7S820: 10,11 THO1: 7,9 TPOX: 8,9 vWA: 16,18 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1 PGM1, 1 PGM3, 2 |
| Name of Depositor |
G Cannon |
| Deposited As |
Homo sapiens |
| Year of Origin |
March, 1974 |
| References |
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
Bellet D, et al. Malignant transformation of nontrophoblastic cells is associated with the expression of chorionic gonadotropin beta genes normally transcribed in trophoblastic cells. Cancer Res. 57: 516-523, 1997. PubMed: 9012484
Bender CM, et al. Inhibition of DNA methylation by 5-Aza-2'-deoxycytidine suppresses the growth of human tumor cell lines. Cancer Res. 58: 95-101, 1998. PubMed: 9426064
Hu SX, et al. Development of an adenovirus vector with tetracycline-regulatable human tumor necrosis factor alpha gene expression. Cancer Res. 57: 3339-3343, 1997. PubMed: 9269991
Schnier JB, et al. G1 arrest and down-regulation of cyclin E/cyclin-dependent kinase 2 by the protein kinase inhibitor staurosporine are dependent on the retinoblastoma protein in the bladder carcinoma cell line 5637. Proc. Natl. Acad. Sci. USA 93: 5941-5946, 1996. PubMed: 8650198
|
| Cross References |
Nucleotide (GenBank) :
E01423
DNA sequence of wild type and mutagenized human G-CSF.
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