| 產(chǎn)品名稱 |
703D4 |
| 商品貨號(hào) |
B163807 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Cancer,Cancer |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts with human non-small cell lung cancer cells but not with small cell lung cancer. The antibody also reacts with human melanomas but does not bind to a variety of other human tumors or normal human tissues. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human lung cancer |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human lung cancer |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with NCI-H157 cells (human large cell lung cancer. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with human non-small cell lung cancer cells but not with small cell lung cancer. The antibody also reacts with human melanomas but does not bind to a variety of other human tumors or normal human tissues. |
| Complete Growth Medium |
Modified Dulbecco's medium with 0.1 mM hypoxanthine, 40 nM aminopterin and 0.016 mM thymidine, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a; kappa light chain |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Mulshine JL, et al. Monoclonal antibodies that distinguish non-small cell from small cell lung cancer. J. Immunol. 131: 497-502, 1983. PubMed: 6306102
Mulshine JL, Minna JD. Monoclonal antibodies against non small cell lung cancer. US Patent 4,569,788 dated Feb 11 1986
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