| 產品名稱 |
7-34-1 |
| 商品貨號 |
B163812 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific. |
| Derivation |
Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against swine leucocyte antigen (SLA) a, c and d haplotypes (cytotoxic) |
| Comments |
Animals were immunized with peripheral blood mononuclear cells (PMNC) from SLA c haplotype miniature swine. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody react with the MHC class I antigen of swine, and is probably heavy chain specific. It does not cross-react with PMNC from humans, sheep, goats or mice, but does cross-react with cells from commercial varieties of pigs. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
CM Warner |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Lie WR, et al. Preparation and characterization of murine monoclonal antibodies to swine lymphocyte antigens. Immunology 64: 599-605, 1988. PubMed: 2459049
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