| Applications |
The hybridoma cell line 807.31 secretes a mouse monoclo-nal antibody (IgG1) reactive with the G1 envelope glycoprotein of La Crosse Virus. This antibody as well as 807.15 (ATCC CRL-2287) and 807.33 (ATCC CRL-2290) is useful for identifying infection by La Crosse virus and other California serogroup Bunyaviruses. The 807.31 antibody has been used in ELISA, Western Blot, immunoprecipitation, immunofluorescence and neutralization assays. The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus. |
| Comments |
The hybridoma cell line 807.31 secretes a mouse monoclo-nal antibody (IgG1) reactive with the G1 envelope glycoprotein of La Crosse Virus. This antibody as well as 807.15 (ATCC CRL-2287) and 807.33 (ATCC CRL-2290) is useful for identifying infection by La Crosse virus and other California serogroup Bunyaviruses. The 807.31 antibody has been used in ELISA, Western Blot, immunoprecipitation, immunofluorescence and neutralization assays. The antibody does not react with the G2 or L proteins of La Crosse virus. The line was produced by fusing P3X63Ag8.653 myeloma cells with spleen cells from BALB/c mice that had been immunized with La Crosse Virus. |
| References |
Gonzalez-Scarano F, et al. Characterization of monoclonal antibodies against the G1 and N proteins of LaCrosse and Tahyna, two California serogroup bunyaviruses. Virology 120: 42-53, 1982. PubMed: 7101727
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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