| 產品名稱 |
8A3B.6 |
| 商品貨號 |
B163843 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for the structural protein VP7. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus |
| Cellular Products |
immunoglobulin; monoclonal antibody; against VP7 of bluetongue virus |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with bluetongue virus (serotype 17). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody is specific for the structural protein VP7. |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 6 X 10 exp4 cells/ml and maintain between 5 X 10 exp4 and 4 X 10 exp5 cells/ml. |
| Isotype |
IgG2a |
| Name of Depositor |
JA House |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Appleton JA, Letchworth GJ. Monoclonal antibody analysis of serotype-restricted and unrestricted bluetongue viral antigenic determinants. Virology 124: 286-299, 1983. PubMed: 6186076
House C, et al. Detection of bluetongue group-specific antibody by competitive ELISA. J. Vet. Diagn. Invest. 2: 137-139, 1990. PubMed: 1965579
|
