Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Applications
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Derivation
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
Genes Expressed
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Cellular Products
immunoglobulin; monoclonal antibody; against bovine herpesvirus 1 (BHV-1)
Comments
Animals were immunized with live BHV-1.
Lymph node cells were fused with Sp2/0 mouse myeloma cells.
Initial hybrids were refused with additional lymph node cells from the same animal and reselected.
The antibody neutralizes BHV-1 and is protective against BHV-1 induced mortality in rabbits.
Complete Growth Medium
Culture Medium: Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 90%; horse serum, 10%.
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Levings RL, Stoll IR. Bovine monoclonal antibodies to bovine herpesvirus I from sequential fusion heterohybridomas. US Patent 5,026,646 dated Jun 25 1991
