| 產品名稱 |
AP-3 |
| 商品貨號 |
B163951 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Leukemia |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts specifically with GPIIIa, but does not inhibit platelet aggregation. It is useful for studies of human GPIIIa expression by flow cytometry, Western blot (non-reduced) and affinity chromatography methods. The antibody can also be used for the classification of human megakaryocytic leukemias and Glanzmann thrombasthenia. |
| Derivation |
Animals were immunized with partially purified human platelet glycoproteins IIb and IIIa (GPIIb and GPIIIa, respectively). Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against beta 3 integrin (platelet GPIIIa) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against beta 3 integrin (platelet GPIIIa) |
| Comments |
Animals were immunized with partially purified human platelet glycoproteins IIb and IIIa (GPIIb and GPIIIa, respectively). Spleen cells were fused with NS-1 myeloma cells. The antibody recognizes all known allelic forms of the beta 3 subunit of the integrin superfamily. The two glycoproteins (GPIIb and GPIIIa) together form the platelet binding site for fibrinogen. The antibody reacts specifically with GPIIIa, but does not inhibit platelet aggregation. It is useful for studies of human GPIIIa expression by flow cytometry, Western blot (non-reduced) and affinity chromatography methods. The antibody can also be used for the classification of human megakaryocytic leukemias and Glanzmann thrombasthenia. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
PJ Newman |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Newman PJ, et al. Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3. Blood 65: 227-232, 1985. PubMed: 3155488
Newman PJ, et al. Synergistic action of two murine monoclonal antibodies that inhibit ADP- induced platelet aggregation without blocking fibrinogen binding. Blood 69: 668-676, 1987. PubMed: 3026525
Isenberg WM, et al. Monoclonal antibodies bound to subunits of the integrin GPIIb-IIIa are internalized and interfere with filopodia formation and platelet aggregation. Blood 76: 1564-1571, 1990. PubMed: 2207330
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