| 產品名稱 |
BA-G5 |
| 商品貨號 |
B163991 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with rat cardiac muscle alpha myosin heavy chain but not with rat skeletal muscle myosins. |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Derivation |
Spleen cells were fused with NSO myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against rat cardiac alpha myosin heavy chain |
| Cellular Products |
immunoglobulin; monoclonal antibody; against rat cardiac alpha myosin heavy chain |
| Comments |
Animals were immunized with bovine fetal skeletal muscle myosin. Spleen cells were fused with NSO myeloma cells. The antibody reacts with rat cardiac muscle alpha myosin heavy chain but not with rat skeletal muscle myosins. The cells were deposited on behalf of Dr. Schiaffino by Regeneron Pharmaceuticals, Inc. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 92.5%; DMSO, 7.5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2b , IgG2b |
| Name of Depositor |
S Schiaffino |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Rudnicki MA, et al. Actin and myosin expression during development of cardiac muscle from cultured embryonal carcinoma cells. Dev. Biol. 138: 348-358, 1990. PubMed: 2318340
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