| 產品名稱 |
BC9-E5 |
| 商品貨號 |
B164013 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Spleen cells were fused with NS-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Lewis a (human blood group antigen) |
| Comments |
Mice were immunized with naked Salmonella minnesota to which had been adsorbed neutral glycolipids from human Type A meconium. Spleen cells were fused with NS-1 myeloma cells. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
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| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
WW Young |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Young WW Jr., et al. Characterization of monoclonal antibodies specific for the Lewis a human blood group determinant. J. Biol. Chem. 258: 4890-4894, 1983. PubMed: 6833281
Galanos C, et al. Preparation and properties of antisera against the lipid-A component of bacterial lipopolysaccharides. Eur. J. Biochem. 24: 116-122, 1971. PubMed: 4944989
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