| 產(chǎn)品名稱 |
bcd mab23 |
| 商品貨號 |
B164014 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo). |
| Derivation |
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the bicoid (bcd) protein of Drosophila melanogaster |
| Comments |
Animals were immunized with purified LacZ - bcd fusion protein and boosted with TrpE - bcd fusion protein. Spleen cells were fused with P3-NSI/1-Ag4-1 myeloma cells. The antibody reacts with the maternally encoded bcd protein (a morphogen in the Drosophila embryo). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG1 |
| Name of Depositor |
W Driever |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Driever W, Nusslein-Volhard C. A gradient of bicoid protein in Drosophila embryos. Cell 54: 83-93, 1988. PubMed: 3383244
Driever W, Nusslein-Volhard C. The bicoid protein determines position in the Drosophila embryo in a concentration-dependent manner. Cell 54: 95-104, 1988. PubMed: 3383245
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