| 產品名稱 |
BE(2)-M17 |
| 商品貨號 |
B164019 |
| Organism |
Homo sapiens, human |
| Tissue |
brain; derived from metastatic site: bone marrow |
| Cell Type |
neuroblast |
| Product Format |
frozen |
| Morphology |
neuroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
neuroblastoma |
| Age |
2 years |
| Gender |
male |
| Applications |
This cell line is a suitable transfection host. |
| Derivation |
BE(2)-M17 is a clone of the SK-N-BE(2) neuroblastoma cell line (see ATCC CRL-2271) that was established in November of 1972 from a bone marrow biopsy taken from a 2 year old male with disseminated neuroblastoma after repeated courses of chemotherapy and radiotherapy. |
| Clinical Data |
Patient had repeated courses of chemotherapy and radiotherapy. male |
| Comments |
BE(2)-M17 cells have a reported saturation density of greater than 1 x 106 cells/cm2
The cells grow as multilayering, neuroblastic cells with occasionally long neuritic processes.
As cultures age, cells will aggregate and float. |
| Complete Growth Medium |
The base medium for this cell line is a 1:1 mixture of ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003, and F12 Medium. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium carefully. Add 1-2 mL trypsin (0.25%) - EDTA (0.53mM) solution and allow flasks to remain at room temperature (or incubate at 37°C) until cells detach. Add fresh culture medium, triturate cells and dispense into new culture flasks.
Subcultivation ratio: 1:20 to 1:50 at 1 to 2 week intervals is recommended
Medium Renewal: Every 4 to 7 days. pH should be less than or equal to 7.3. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11 D16S539: 11 D5S818: 12 D7S820: 9,10 THO1: 6 TPOX: 8,11 vWA: 18 |
| Population Doubling Time |
20 to 24 hrs |
| Name of Depositor |
JL Biedler |
| Deposited As |
Homo sapiens |
