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C1.18.4

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編號(hào):B164067

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產(chǎn)品名稱	C1.18.4
商品貨號(hào)	B164067
Organism	Mus musculus, mouse
Cell Type	B lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	plasmacytoma; myeloma
Strain	C3H
Applications	This line was derived from the X-5563 tumor which arose spontaneously in a C3H mouse. Tested and found negative for ectromelia virus (mousepox).
Storage Conditions	liquid nitrogen vapor phase
Karyotype	near tetraploid
Derivation	This line was derived from the X-5563 tumor which arose spontaneously in a C3H mouse.
Antigen Expression	H-2k; PC.1
Genes Expressed	immunoglobulin; monoclonal antibody,H-2k; PC.1
Cellular Products	immunoglobulin; monoclonal antibody
Comments	This line was derived from the X-5563 tumor which arose spontaneously in a C3H mouse. Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	Freeze medium: complete growth medium, 95%; DMSO, 5%
Isotype	IgG2a; kappa light chain
Name of Depositor	K Horibata, AW Harris
Deposited As	Mus musculus
References	Ralph P. Retention of lymphocyte characteristics by myelomas and theta+-lymphomas: sensitivity to cortisol and phytohemagglutinin. J. Immunol. 110: 1470-1475, 1973. PubMed: 4541304 Ralph P, Nakoinz I. Lipopolysaccharides inhibit lymphosarcoma cells of bone marrow origin. Nature 249: 49-51, 1974. PubMed: 4208429 Horibata K, Harris AW. Mouse myelomas and lymphomas in culture. Exp. Cell Res. 60: 61-77, 1970. PubMed: 5439579 Hyman R, et al. Cell-specific antigens and immunoglobulin synthesis of murine myeloma cells and their variants. J. Natl. Cancer Inst. 48: 173-184, 1972. PubMed: 4119881 Watson J, et al. Leukemia viruses associated with mouse myeloma cells. Proc. Natl. Acad. Sci. USA 66: 344-351, 1970. PubMed: 4317914 Schubert D, Horibata K. Immunoglobulin biosynthesis. II. Four independently isolated myeloma variants. J. Mol. Biol. 38: 263-271, 1968. PubMed: 4180612

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