| 產品名稱 |
C171 [Clone 171] |
| 商品貨號 |
B164074 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Strain |
Strain:? BALB/c (B cell); BALB/c (myeloma) |
| Applications |
The antibody reacts equivalently with all 3 isoforms of KTI and binds equally well to KTI - trypsin complexes. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Kunitz soybean trypsin inhibitor |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with Kunitz soybean trypsin inhibitor (KTI). Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts equivalently with all 3 isoforms of KTI and binds equally well to KTI - trypsin complexes. It does not discriminate between native and denatured KTI. |
| Complete Growth Medium |
Culture medium: RPMI 1640 with 2 mM L-glutamine, 1 mM sodium pyruvate and
10 mM HEPES, 85%; horse serum, 15%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
culture medium 95%; DMSO, 5% |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG1; kappa light chain |
| Name of Depositor |
The United States of America |
| U.S. Patent Number |
|
| References |
Brandon DL, et al. Monoclonal antibodies to soybean Kunitz trypsin inhibitor and immunoassay methods. US Patent 4,959,310 dated Sep 25 1990
Brandon DL, et alAntigenicity of soybean protease inhibitorsIn: Brandon DL, et alProtease inhibitors as cancer chemopreventive agentsNew YorkPlenum Presspp. 107-129, 1993
. . J. Food Sci. 53: 97-101, 1988.
J. Agric. Food Chem. 36: 1336-1341, 1988.
. . Crop Sci. 32: 1502-1505, 1992.
J. Agric. Food Chem. 39: 327-335, 1991.
|
