| 產品名稱 |
C273 |
| 商品貨號 |
B164079 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
C273 is was derived from the Sp2/0 hybridoma cell line. The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
C273 is was derived from the Sp2/0 hybridoma cell line. The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human CD20 (chimeric immunoglobulin)) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human CD20 (chimeric immunoglobulin)) |
| Comments |
C273 is was derived from the Sp2/0 hybridoma cell line. It is transfected with plasmids pING2101 and pING2106. The cells are neomycin resistant and express chimeric heavy and light chain antibody genes encoding the variable region of the mouse monoclonal antibody produced by 2H7 and a human constant region. |
| Complete Growth Medium |
Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and supplemented with 0.05 mM 2-mercaptoethanol, 80%; fetal bovine serum, 20%
|
| Subculturing |
Medium Renewal: Every 3 to 4 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Xoma Corporation |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Robinson RR, et al. Chimeric antibody with specificity to human B cell surface antigen. US Patent 5,500,362 dated Mar 19 1996
Clark EA, et al. Role of the Bp35 cell surface polypeptide in human B-cell activation. Proc. Natl. Acad. Sci. USA 82: 1766-1770, 1985. PubMed: 3872456
Profous-Juchelka H, et al. Recombinant and native group B eimeria tenella immunogens useful as coccidiosis vaccines. US Patent 5,824,656 dated Oct 20 1998
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