| 產品名稱 |
Calu-1 |
| 商品貨號 |
B164113 |
| Organism |
Homo sapiens, human |
| Tissue |
lung; derived from metastatic site: pleura |
| Product Format |
frozen |
| Morphology |
epithelial |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
grade III,epidermoid carcinoma |
| Age |
47 years |
| Gender |
male |
| Ethnicity |
Caucasian |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
The stemline chromosome number is hypotriploid and the 2S component occurred at 14.2%. Modal chromosome number is 62. Seven markers occurred frequently, M1 (two copies per cell), M6 and M7 were found in most cells; M2 and M3, and M4 and M5 appeared to be mutually exclusive, i.e., only one of M2 or M3, and one of M4 or M5 were present in each cell. A Y chromosome was not detected by QM band examination, although the cell line was initiated from a male. |
| Clinical Data |
47 years Caucasian male |
| Antigen Expression |
Antigen expression: Blood Type A; Rh+; HLA A10, A11, B15, Bw35 |
| Tumorigenic |
Yes |
| Effects |
Yes, in nude mice; forms epidermoid carcinomas Yes, in steroid treated hamsters |
| Comments |
Ultrastructural features include numerous microvilli, prominent RER, lysosomes, lipid inclusions, no virus particles. Contains the ras (K-ras) oncogene. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Interval: every 6 to 8 days
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: culture medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 11,12 D16S539: 11 D5S818: 10,12 D7S820: 9,10 THO1: 9,9.3 TPOX: 8 vWA: 15,16 |
| Isoenzymes |
AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 Me-2, 1-2 PGM1, 1-2 PGM3, 1 |
| Name of Depositor |
J Fogh |
| Deposited As |
Homo sapiens |
| References |
Fogh J. Human tumor cells in vitro. New York: Plenum Press; 1975.
Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 833871
Goodfellow M, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 77210034
Tew KD, Taylor DM. Studies with cyclophosphamide labeled with phosphorus-32: nucleic acid alkylation and its effect on DNA synthesis in rat tumor and normal tissues. J. Natl. Cancer Inst. 58: 1413-1418, 1977. PubMed: 857031
Fogh J. Cultivation, characterization, and identification of human tumor cells with emphasis on kidney, testis, and bladder tumors. Natl. Cancer Inst. Monogr. 49: 5-9, 1978. PubMed: 571047
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