| 產品名稱 |
CC51 |
| 商品貨號 |
B164135 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody is specific for a 145000 dalton molecule that is the bovine analogue of human CD21. |
| Derivation |
Spleen cells were fused with NS1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against bovine CD21 (BoCD21) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against bovine CD21 (BoCD21) |
| Comments |
Animals were immunized with mesenteric lymph node cells from a Friesian cow. Spleen cells were fused with NS1 myeloma cells. The antibody is specific for a 145000 dalton molecule that is the bovine analogue of human CD21. The antigen is expressed on bovine B cells and not on bovine T cells. |
| Complete Growth Medium |
Culture Medium: RPMI medium 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
Culture medium, 95%; DMSO, 5% |
| Isotype |
IgG2b |
| Name of Depositor |
CJ Howard |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Naessens J, et al. Characterization of a bovine leucocyte differentiation antigen of 145,000 MW restricted to B lymphocytes. Immunology 69: 525-530, 1990. PubMed: 2185984
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