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• fetal bovine serum 5% (final conc.)
• NCTC 109 (InVitrogen Cat No. 21340-039) 5% (final conc.)
• HL-1 Supplement (HL-1 medium supplement Lonza Cat No. 77227 or 77228) 1% (final conc.)
• 0.1 mM hypoxanthine
• 0.016 mM thymidine

Complete growth medium described above supplemented with 5% (v/v) DMSO.  Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.

Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.

Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.