| Applications |
The hybridoma cell line DAL K20 secretes a mouse monoclonal antibody (IgG1) reactive with the lining epithelium of human normal proximal and distal convoluted tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis. This antibody as well as DAL K29 (ATCC CRL-2291) and DAL 45 (ATCC CRL-2292) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| Comments |
The hybridoma cell line DAL K20 secretes a mouse monoclonal antibody (IgG1) reactive with the lining epithelium of human normal proximal and distal convoluted tubules, renal cell carcinoma and the basal layer of fetal or neonatal epidermis. This antibody as well as DAL K29 (ATCC CRL-2291) and DAL 45 (ATCC CRL-2292) is useful for identifying kidney differentiation antigens and has been shown effective in radioimaging as well as tumor growth inhibition in xenograft models. The line was produced by fusing SP2 myeloma cells with spleen cells from BALB/c mice that had been immunized with the Caki-1 (ATCC HTB-46) clear cell kidney carcinoma cell line. |
| References |
Luner SJ, et al. Monoclonal antibodies to kidney and tumor-associated surface antigens of human renal cell carcinoma. Cancer Res. 46: 5816-5820, 1986. PubMed: 3530441
Guha AK, et al. Tumor localization of monoclonal antibodies against human renal carcinoma in a xenograft model. Cancer Lett. 61: 35-43, 1991. PubMed: 1764696
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
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