| 產品名稱 |
DBS-FRhL-2 |
| 商品貨號 |
B164337 |
| Organism |
Macaca mulatta, monkey, rhesus |
| Tissue |
lung |
| Cell Type |
fibroblast |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Age |
fetus |
| Gender |
male |
| Storage Conditions |
liquid nitrogen vapor phase |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype |
Model number = 42. Range = 37 to 46.Seventy percent of the cells are diploid and the karyotype is that of a male rhesus monkey. No structural abnormalities are evident. |
| Clinical Data |
male |
| Tumorigenic |
No |
| Effects |
No, in immunosuppressed mice Yes, in semisolid medium. no; The cells were not tumorigenic in immunosuppressed mice, but did form colonies in semisolid medium. |
| Virus Susceptibility |
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Human Coxsackievirus A 9
Parainfluenza 3
Rubella virus
,
Rubella virus
Mumps rubulavirus
,
Mumps virus
Vaccinia virus
Vesicular stomatitis, Orsay (Indiana)
Vesicular stomatitis, Glasgow (Indiana)
Human rhinovirus 2
|
| Comments |
This line was formerly designated CCL-160. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
-
Remove and discard culture medium.
-
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
-
Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes). Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
-
Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
-
Add appropriate aliquots of the cell suspension to new culture vessels.
-
Incubate cultures at 37°C.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
U.S. Government |
| Deposited As |
Macaca mulatta |
| U.S. Patent Number |
|
| Year of Origin |
April, 1969 |
| References |
Hopps HE, et al. Sub-human primate diploid cell lines as substrates for virus vaccine production. US Patent 4,040,905 dated Aug 9 1977
Wallace RE, et al. Development of a diploid cell line from fetal rhesus monkey lung for virus vaccine production. In Vitro 8: 323-332, 1973. PubMed: 4348695
|
