| Comments |
The cell express HLA class I and class II antigens.
Derivative chromosomes observed at high frequency in 24 karyotyped metaphases included: der(2)t(2;?18)(q10;q10); del(3)?(pter-->p21::q10-->qter); del(6)(q13); der(10); add(11)(p15); der(14)t(14;17)(q10;q10); add(15)(p11); add(15)(p11)+; and del(17)(?q24).
Two copies of each derivative chromosome was observed in each metaphase.
Less frequent derivative chromosomes included: del(12)?(q11q21)[18/24 cells] and add(13)(p11)[10/24 cells]. Four to eight additional marker chromosomes of unknown origin were generally detected in each cell.
Cytogenetics Comments: This is a hyper-triploid human cancer cell line with a modal chromosome number of 79.
A Y chromosome was verified by QM staining and C-banding. Normal chromosomes 10, 15 and 17 were absent.
The composite karyotype consists of 65 to 84, XY, +1, -2, der(2)t(2;?18)(q10;q10)(x2); -3, del(3)?(pter-->p21::q10-->qter)x2, -4, -5, +6, del(6)(?q15)x2,+7,+8,-9,-10,-10,-10, der(10)x2, -11, add(11)(p15)x2, -12, del(12)?(q11q21)[18], -13, +14, der(14)t(14;17)(q10;q10)x2, -15, -15,- 15, der(15)x2, -16, -17, -17, 17, del(17)x2, -18, +19, -20, +21, -22[cp24] |
| References |
Bepler G, et al. Expression of p64c-myc and neuroendocrine properties define three subclasses of small cell lung cancer. Oncogene 4: 45-50, 1989. PubMed: 2536917
Pettengill OS, et al. Isolation and growth characteristics of continuous cell lines from small-cell carcinoma of the lung. Cancer 45: 906-918, 1980. PubMed: 6266631
Pettengill OS, et al. Animal model for small cell carcinoma of the lung. Effect of immunosuppression and sex of mouse on tumor growth in nude athymic mice. Exp. Cell Biol. 48: 279-297, 1980. PubMed: 6249655
Lung Cancer 4: 155-161, 1988.
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