| 產品名稱 |
E13 161-7 |
| 商品貨號 |
B164387 |
| Organism |
Rattus norvegicus (B cell); Mus musculus (myeloma), rat (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with an antigen expressed on mouse hematopoietic stem cells. Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells. |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
Spleen cells were fused with P3X63 myeloma cells. |
| Genes Expressed |
It cross-reacts with epithelial elements in human kidney, tonsils, pancreatic ducts, lymph nodes and esophagus. immunoglobulin; monoclonal antibody; against mouse stem cell antigen 1 (Sca-1),Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells. |
| Cellular Products |
immunoglobulin; monoclonal antibody; against mouse stem cell antigen 1 (Sca-1) |
| Comments |
Animals were immunized with a pre-T cell hybridoma. Spleen cells were fused with P3X63 myeloma cells. The antibody reacts with an antigen expressed on mouse hematopoietic stem cells. Among bone bone marrow cells that are negative for lineage specific markers and have low expression of the Thy-1 antigen, the Sca-1 antigen identifies a virtually pure population of multilineage hematopoietic stem cells. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium:
- fetal bovine serum to a final concentration of 10%
- 0.05 mM 2-mercaptoethanol
|
| Subculturing |
Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37.0°C |
| Isotype |
IgG2a , IgG2a |
| Name of Depositor |
IL Weissman |
| Deposited As |
rat (B cell); mouse (myeloma) |
| References |
Aihara Y, et al. An attempt to produce ``pre-T'' cell hybridomas and to identify their antigens. Eur. J. Immunol. 16: 1391-1399, 1986. PubMed: 3096743
Spangrude GJ, et al. Purification and characterization of mouse hematopoietic stem cells. Science 241: 58-62, 1988. PubMed: 2898810
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