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EL4.BU

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編號:B164405

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產品名稱	EL4.BU
商品貨號	B164405
Organism	Mus musculus, mouse
Cell Type	T lymphocyte
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	lymphoma
Strain	C57BL/6N
Applications	This line was derived from the EL4 cell line (see ATCC TIB-39). The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine (BUdR). Tested and found negative for ectromelia virus (mousepox).
Storage Conditions	liquid nitrogen vapor phase
Derivation	This line was derived from the EL4 cell line (see ATCC TIB-39).
Antigen Expression	H-2b; Thy-1.2
Genes Expressed	H-2b; Thy-1.2
Comments	This line was derived from the EL4 cell line (see ATCC TIB-39). The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine (BUdR). Tested and found negative for ectromelia virus (mousepox).
Complete Growth Medium	Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
Subculturing	Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cryopreservation	Freeze medium: complete growth medium, 95%; DMSO, 5%
Culture Conditions	Temperature: 37.0°C Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Name of Depositor	R Hyman
Deposited As	Mus musculus
References	Mohit B, Fan K. Hybrid cell line from a cloned immunoglobulin-producing mouse myeloma and a nonproducing mouse lymphoma. Science 171: 75-77, 1971. PubMed: 4099030 Gehring U, et al. Glucocorticoid action on hybrid clones derived from cultured myeloma and lymphoma cell lines. Proc. Natl. Acad. Sci. USA 69: 3124-3127, 1972. PubMed: 4343956 Kohler G, et al. Fusion of T and B cells. Somatic Cell Genet. 3: 303-312, 1977. PubMed: 305123 Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.

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