| 產品名稱 |
EL4.BU.1.OUAr.1.1 |
| 商品貨號 |
B164406 |
| Organism |
Mus musculus, mouse |
| Cell Type |
T lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
lymphoma |
| Strain |
C57BL/6N |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
This line was derived from the EL4 cell line (see ATCC TIB-39). |
| Antigen Expression |
H-2b; Thy-1.2 |
| Comments |
This line was derived from the EL4 cell line (see ATCC TIB-39). The cells are resistant to 0.1 mM 5-bromo-2'-deoxyuridine and 1 mM ouabain. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium, 90%; horse serum, 10%
|
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2-3 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days
|
| Culture Conditions |
Temperature: 37°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5% |
| Name of Depositor |
R Hyman |
| Deposited As |
Mus musculus |
| References |
Hyman R, Stallings V. Characterization of a TL- variant of a homozygous TL+ mouse lymphoma. Immunogenetics 3: 75-84, 1976.
|
