| 產(chǎn)品名稱 |
ES-2 |
| 商品貨號 |
B164416 |
| Organism |
Homo sapiens, human |
| Tissue |
ovary |
| Product Format |
frozen |
| Morphology |
fibroblast |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
clear cell carcinoma |
| Age |
47 years |
| Gender |
female |
| Ethnicity |
Black |
| Storage Conditions |
liquid nitrogen vapor phase |
| Karyotype |
This line has a complex hyperdiploid karyotype of 66XX to 88XX. |
| Derivation |
The ES-2 cell line was established from a surgical tumor specimen taken from a 47 year old black woman. |
| Clinical Data |
47 years
Black
female
|
| Genes Expressed |
P glycoprotein |
| Cellular Products |
P glycoprotein |
| Tumorigenic |
Yes |
| Effects |
Yes, tumors developed within 21 days at 100% frequency (5/5) in nude mice inoculated subcutaneously with 107 cells. |
| Comments |
The tumor was described as a poorly differentiated ovarian clear cell carcinoma.
Initially, the cells were grown in soft agar.
The cells exhibit low to moderate resistance to a number of chemotherapeutic agents including doxorubicin, cisplatin, carmustine, etoposide and cyanomorpholinodoxorubicin (MRA-CN).
ES-2 cells express low levels of P glycoprotein. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Remove spent medium, add fresh EDTA solution (0.15 g disodium EDTA, 4.0 g NaCL, 0.28 g sodium bicarbonate, 0.5 g dextrose and 0.2 g KCl dissolved in 500 mL double distilled water). Allow the cells to sit at room temperature for a few minutes, and dislodge the cells by rapping the side of the flask sharply with the palm of your hand. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week |
| Cryopreservation |
Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage Temperature: Liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10,15 D13S317: 11 D16S539: 11,13 D5S818: 11,13 D7S820: 11 THO1: 9.3 TPOX: 8,12 vWA: 16,17 |
| Population Doubling Time |
24 hrs |
| Name of Depositor |
BI Sikic |
| Deposited As |
Homo sapiens |
| References |
Lau DH, et al. Multifactorial mechanisms associated with broad cross-resistance of ovarian carcinoma cells selected by cyanomorpholino doxorubicin. Cancer Res. 51: 5181-5187, 1991. PubMed: 1717140
|
