| 產(chǎn)品名稱 |
F5-A-1/22.8.13 |
| 商品貨號(hào) |
B164430 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified PA. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human prostate antigen (PA) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human prostate antigen (PA) |
| Comments |
Animals were immunized with purified PA. Spleen cells were fused with P3X63Ag8 myeloma cells. |
| Complete Growth Medium |
Culture medium: RPMI 1640, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
fetal bovine serum, 90%; DMSO, 10% |
| Isotype |
IgM; kappa light chain |
| Name of Depositor |
Research Corporation |
| U.S. Patent Number |
|
| References |
Wang MC, et al. Purified human prostate antigen. US Patent 4,446,122 dated May 1 1984
Wang MC, et al. Purification of a human prostate specific antigen. Invest. Urol. 17: 159-163, 1979. PubMed: 89106
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