| 產(chǎn)品名稱(chēng) |
FDC-P1 |
| 商品貨號(hào) |
B164449 |
| Organism |
Mus musculus, mouse |
| Tissue |
bone marrow |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
Normal |
| Strain |
DBA/2 |
| Applications |
This cell line is a suitable transfection host. |
| Storage Conditions |
liquid nitrogen vapor temperature |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Karyotype |
diploid |
| Derivation |
The FDC-P1 cell line was established from long term culture of normal DBA/2 bone marrow cells in medium conditioned by WEHI-3 cells (see ATCC TIB-68). |
| Antigen Expression |
Thy-1.2 (24%) |
| Comments |
They are dependent upon WEHI-3 conditioned medium (WEHI-3CM) for continued growth. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium:
- mouse Interleukin-3 culture supplement (BD Biosciences, Catalog No. 354040) 25% (final conc.)
- fetal bovine serum 10% (final conc.)
To make a 500 mL volume:
- 325 mL DMEM (ATCC 30-2002)
- 125 mL Mouse Interleukin-3 (BD 354040)
- 50 mL FBS (ATCC 30-2020)
This medium is formulated for use with a 5% CO2 in air atmosphere. Standard DMEM formulations contain 3.7 g/L sodium bicarbonate. A 10% CO2 in air atmosphere is recommended. |
| Subculturing |
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 105 cells/mL and maintain between 1 X 105 and 1 X 106 cells/mL.
Medium Renewal: Every 2 to 3 days |
| Cryopreservation |
Freeze medium: Complete growth medium, 90%; DMSO, 10% Storage temperature: liquid nitrogen vapor temperature |
| Culture Conditions |
Temperature: 37°C |
| Name of Depositor |
University of Virginia Patent Foundation |
| Deposited As |
Mus musculus |
| U.S. Patent Number |
|
| References |
Dexter TM, et al. Growth of factor-dependent hemopoietic precursor cell lines. J. Exp. Med. 152: 1036-1047, 1980. PubMed: 6968334
|
