| 產(chǎn)品名稱 |
gamma3-11.1 |
| 商品貨號 |
B164492 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Spleen cells were fused with P3X63Ag8.U1 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against human gamma interferon |
| Cellular Products |
immunoglobulin; monoclonal antibody; against human gamma interferon |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in BALB/c mice |
| Comments |
Animals were immunized with a synthetic peptide (NH2-Lys-Arg-Lys-Arg- Ser-Gln-Met-Leu-Phe-Arg-Gly-Arg-Arg-Ala-Ser-Gln-COOH) conjugated to thyroglobulin. Spleen cells were fused with P3X63Ag8.U1 myeloma cells. |
| Complete Growth Medium |
Culture Medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain
1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium
pyruvate, 90%; fetal bovine serum, 10%. |
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
Takeda Chem. Ind., Ltd. |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| U.S. Patent Number |
|
| References |
Ichimori Y, et al. Novel peptide and use thereof. US Patent 4,681,848 dated Jul 21 1987
|
