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H-4-II-E

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編號:B164539

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產(chǎn)品名稱	H-4-II-E
商品貨號	B164539
Organism	Rattus norvegicus, rat
Tissue	liver
Product Format	frozen
Morphology	epithelial
Culture Properties	adherent
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	hepatoma
Applications	The line is useful for detecting picogram quantities of planar polychlorinated organic compounds in environmental samples and food extracts.
Genes Expressed	-
Cellular Products	aryl hydrocarbon hydroxylase is inducible
Comments	The line has been cured of Mycoplasma arginini contamination.
Complete Growth Medium	The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing	Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks. Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended Medium Renewal: Every 2 to 3 days
Cryopreservation	culture medium 95%; DMSO, 5%
Culture Conditions	Temperature: 37°C
Name of Depositor	JA Bradlaw
Deposited As	Rattus sp.
U.S. Patent Number	4,865,972
References	Benedict WF, et al. Aryl hydrocarbon hydroxylase induction in mammalian cell culture--IV. Stimulation of the enzyme activity in established cell lines derived from rat or mouse hepatoma and from normal rat liver. Biochem. Pharmacol. 22: 2766-2769, 1973. PubMed: 4357894 . . In Vitro 16: 230, 1980. Sassier P, Bergeron M. Specific inhibition of cell proliferation in the mouse intestine by an aqueous extract of rabbit small intestine. Cell Tissue Kinet. 10: 223-243, 1977. PubMed: 872181 Bradlaw JA, Casterline JL Jr.. Induction of enzyme activity in cell culture: a rapid screen for detection of planar polychlorinated organic compounds. J. Assoc. Off. Anal. Chem. 62: 904-916, 1979. PubMed: 115836 Peraino C, et al. Hepatomas in tissue culture compared with adapting liver in vivo. Natl. Cancer Inst. Monogr. 13: 229-245, 1964. PubMed: 14143233
Cross References	Nucleotide (GenBank) : U49128 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3b (delta part IVS4), partial cds. Nucleotide (GenBank) : U49126 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta IVS4), partial cds. Nucleotide (GenBank) : U49127 Rattus norvegicus neuroendocrine calcium channel alpha-1 subunit mRNA, variant CaCh3 (delta IVS3 delta part IVS4), partial cds.

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