| Derivation |
HCC1007BL is a B lymphoblastoid cell line initiated by Epstein-Barr virus (EBV) transformation of peripheral blood lymphocytes obtained from the same patient as the breast carcinoma cell line HCC1008 (ATCC CRL-2320).
HCC1008 was initiated from a 67 year old Black female with a TNM stage IIA, grade 3 metastatic carcinoma; metastatic site: lymph node.
|
| Subculturing |
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternately, cultures can be established by centrifugation with subsequent resuspension at 3 to 5 X 105 viable cells/mL. Maintain cell density between 3 X 105 and 1 X 106 viable cells/mL.
Medium Renewal: Add fresh medium every 3 to 4 days (depending on cell density) |
| References |
Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771
Hay, R. J., Caputo, J. L., and Macy, M. L., Eds. (1992), ATCC Quality Control Methods for Cell Lines. 2nd edition, Published by ATCC.
Caputo, J. L., Biosafety procedures in cell culture. J. Tissue Culture Methods 11:223-227, 1988.
Fleming, D.O., Richardson, J. H., Tulis, J.J. and Vesley, D., (1995) Laboratory Safety: Principles and Practice. Second edition, ASM press, Washington, DC.
Biosafety in Microbiological and Biomedical Laboratories, 5th ed. HHS. U.S. Department of Health and Human Services, Centers for Disease Control and Prevention. Washington DC: U.S. Government Printing Office; 2007. The entire text is available online.
|
