| 產品名稱 |
HCC1954 |
| 商品貨號 |
B164568 |
| Organism |
Homo sapiens, human |
| Tissue |
mammary gland; breast/duct |
| Cell Type |
Epithelial |
| Product Format |
frozen |
| Morphology |
large epithelial cells with occasional vacuoles |
| Culture Properties |
adherent |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Disease |
TNM stage IIA, grade 3, ductal carcinoma |
| Age |
61 years adult |
| Gender |
female |
| Ethnicity |
East Indian |
| Storage Conditions |
liquid nitrogen vapor phase |
| Derivation |
HCC1954 was derived from a primary stage IIA, grade 3 invasive ductal carcinoma with no lymph node metastases. The HCC1954 is a poorly differentiated cell line initiated on October 30, 1995; it took about 4 months to establish. |
| Clinical Data |
61 years adult East Indian female |
| Receptor Expression |
estrogen receptor, not expressed progesterone receptor, not expressed |
| Oncogene |
her2/neu + (overexpressed) |
| Genes Expressed |
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Cellular Products |
Epithelial glycoprotein 2 [EGP2]; cytokeratin 19 |
| Comments |
HCC1954 is positive for the epithelial cell specific marker Epithelial Glycoprotein 2 and for cytokeratin 19, and is negative for expression of estrogen receptor (ER) and progesterone receptor (PR).
Her2/neu is overexpressed in the ELISA assay.
|
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
- Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels.
- Incubate cultures at 37°C
Subculture Ratio: 1:4 to 1:8
Medium Renewal: 2 to 3 times a week.
Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 10 in Culture of Animal Cells, a manual of Basic Technique by R. Ian Freshney, 3rd edition, published by Alan R. Liss, N.Y., 1994. |
| Cryopreservation |
Freeze medium: Complete growth medium 95%; DMSO, 5% Storage temperature: liquid nitrogen vapor phase |
| Culture Conditions |
Temperature: 37°C |
| STR Profile |
Amelogenin: X CSF1PO: 10 D13S317: 8,9 D16S539: 9,11 D5S818: 11 D7S820: 10,11 THO1: 6,7 TPOX: 8,9 vWA: 18,19 |
| Name of Depositor |
AF Gazdar, AK Virmani |
| Year of Origin |
October 30, 1995 |
| References |
Gazdar AF, et al. Characterization of paired tumor and non-tumor cell lines established from patients with breast cancer. Int. J. Cancer 78: 766-774, 1998. PubMed: 9833771
|
