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HEL 92.1.7

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編號:B164590

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產品名稱	HEL 92.1.7
商品貨號	B164590
Organism	Homo sapiens, human
Tissue	bone marrow
Cell Type	erythroblast
Product Format	frozen
Morphology	lymphoblast
Culture Properties	suspension
Biosafety Level	1 Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease	erythroleukemia
Age	30 years
Gender	male
Ethnicity	Caucasian
Storage Conditions	liquid nitrogen vapor phase
Clinical Data	30 years Caucasian male
Antigen Expression	Antigen expression: HLA A3, Aw32, Bw35; Ia+
Genes Expressed	hemoglobin; globin (G gamma, A gamma, epsilon, eta and alpha chains); beta-2-microglobulin; glycophorin
Comments	These cells differentiate spontaneously into erythroblast-like cells. Macrophage-like differentiation can be induced with phorbol esters such as TPA (12-O-tetradecanoyl-phorbol-13-acetate) and PMA (phorbol myristic acid).
Complete Growth Medium	The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
Subculturing	Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 x 10⁵ cells/mL and maintain between 1 x 10⁵ and 1 x 10⁶ cells/mL. Medium Renewal: Every 2 to 3 days
STR Profile	Amelogenin: X,Y CSF1PO: 10 D13S317: 9,11 D16S539: 11 D5S818: 11 D7S820: 7 THO1: 7 TPOX: 11 vWA: 14,17
Name of Depositor	PJ Martin
Deposited As	Homo sapiens
References	Papayannopoulou T, et al. Human erythroleukemia cell line (HEL) undergoes a drastic macrophage- like shift with TPA. Blood 62: 832-845, 1983. PubMed: 6309287 Martin P, Papayannopoulou T. HEL cells: a new human erythroleukemia cell line with spontaneous and induced globin expression. Science 216: 1233-1235, 1982. PubMed: 6177045 Clark RA, et al. Tenascin supports lymphocyte rolling. J. Cell Biol. 137: 755-765, 1997. PubMed: 9151679 Gan W, Rhoads RE. Internal initiation of translation directed by the 5'-untranslated region of the mRNA for eIF4G, a factor involved in the picornavirus-induced switch from cap-dependent to internal initiation. J. Biol. Chem. 271: 623-626, 1996. PubMed: 8557663 Riewald M, et al. Identification and characterization of the cytoplasmic antiproteinase (CAP) in human platelets. J. Biol. Chem. 271: 7160-7167, 1996. PubMed: 8636153

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