| 產品名稱 |
HP6014 |
| 商品貨號 |
B164633 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region. This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Derivation |
Animals were immunized with purified human immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the Fd portion of human IgG2 (Fab region) |
| Tumorigenic |
Yes |
| Effects |
Yes, in BALB/c mice |
| Comments |
Animals were immunized with purified human immunoglobulin. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The HP6014 antibody represents the only known IgG2 subclass specific reagent for the Fab region. This antibody was selected as a reference reagent of the WHO Collaborating Center at the Centers for Disease Control for Human Immunoglobulin Subclasses. Tested and found negative for ectromelia virus (mousepox). |
| Complete Growth Medium |
Dulbecco's modified Eagle's medium with 4.5 g/L glucose, 10 mM HEPES and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
CB Reimer |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Reimer CB, et al. Evaluation of thirty-one mouse monoclonal antibodies to human IgG epitopes. Hybridoma 3: 263-275, 1984. PubMed: 6209201
Jefferis R, et al. Evaluation of monoclonal antibodies having specificity for human IgG sub-classes: Results of an IUIS/WHO collaborative study. Immunol. Lett. 10: 223-252, 1985. PubMed: 3899923
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