Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country.
Disease
cancer, adenocarcinoma
Applications
The antibody reacts with human colon adenocarcinoma cells.
This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers.
Disclosure
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC.
Genes Expressed
immunoglobulin; monoclonal antibody; against human colon adenocarcinoma
Cellular Products
immunoglobulin; monoclonal antibody; against human colon adenocarcinoma
Comments
Animals were immunized with a human colon cancer cell line (HT-29, see ATCC HTB-38).
The antibody reacts with human colon adenocarcinoma cells.
It does not react with frozen sections of normal adult tissues.
This is one of a panel of hybridomas (ATCC HB-8232 and ATCC HB-8240 through ATCC HB-8252) that can be used to diagnose and classify human gastrointestinal tract cancers.
Complete Growth Medium
RPMI 1640 medium with non-essential amino acids and 2% HT, 90%; fetal bovine serum, 10%
Subculturing
Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Cordon-Cardo C, et al. Monoclonal antibodies to human gastrointestinal cancers and hybridoma method of production of the monoclonal antibodies. US Patent 4,579,827 dated Apr 1 1986
