| 產(chǎn)品名稱(chēng) |
IgG-5D7 [anti LRP 515] |
| 商品貨號(hào) |
B164843 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts specifically with the 515 kDa subunit of rabbit LRP. |
| Derivation |
Animals were immunized with purified rabbit LRP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against the 515000 dalton (515 kDa) subunit of rabbit lipoprotein receptor related protein (LRP) |
| Cellular Products |
immunoglobulin; monoclonal antibody; against the 515000 dalton (515 kDa) subunit of rabbit lipoprotein receptor related protein (LRP) |
| Comments |
Animals were immunized with purified rabbit LRP. Spleen cells were fused with Sp2/0-Ag14 myeloma cells. The antibody reacts specifically with the 515 kDa subunit of rabbit LRP. It does not react with the 85 kDa subunit of rabbit LRP, nor does it cross-react with the 515 kDa subunit of rat LRP. |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: 1 to 2 times per week Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Isotype |
IgG1 |
| Name of Depositor |
YK Ho, JL Goldstein |
| Deposited As |
mouse (B cell); mouse (myeloma) |
| References |
Herz J, et al. Low density lipoprotein receptor-related protein mediates endocytosis of monoclonal antibodies in cultured cells and rabbit liver. J. Biol. Chem. 265: 21355-21362, 1990. PubMed: 2250029
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