| 產品名稱 |
J5-1 |
| 商品貨號 |
B164876 |
| Organism |
Mus musculus (B cell); Mus musculus (myeloma), mouse (B cell); mouse (myeloma) |
| Cell Type |
hybridoma: B lymphocyte |
| Product Format |
frozen |
| Morphology |
lymphoblast |
| Culture Properties |
suspension |
| Biosafety Level |
1
Biosafety classification is based on U.S. Public Health Service Guidelines, it is the responsibility of the customer to ensure that their facilities comply with biosafety regulations for their own country. |
| Applications |
The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells. The antibody is protective in mice against Pseudomonas aeruginosa burn infection. |
| Disclosure |
This material is cited in a US or other Patent and may not be used to
infringe the claims. Depending on the wishes of the Depositor, ATCC may be
required to inform the Patent Depositor of the party to which the material was
furnished. This material may not have been produced or characterized by ATCC. |
| Derivation |
Animals were immunized with purified E. coli J5 endotoxin core. Spleen cells were fused with P3X63Ag8.653 myeloma cells. |
| Genes Expressed |
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core |
| Cellular Products |
immunoglobulin; monoclonal antibody; against Escherichia coli J5 endotoxin core |
| Tumorigenic |
Yes |
| Effects |
Yes, forms ascites in pristane primed BALB/c mice |
| Comments |
Animals were immunized with purified E. coli J5 endotoxin core. Spleen cells were fused with P3X63Ag8.653 myeloma cells. The antibody reacts with endotoxins from Gram negative bacteria, and inhibits endotoxin induced mitogenesis of B cells. The antibody is protective in mice against Pseudomonas aeruginosa burn infection. The epitope recognized by J5-1 appears to be distinct from that recognized by J5-2 (ATCC HB-8298). |
| Complete Growth Medium |
The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, ATCC 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum (ATCC 30-2020) to a final concentration of 10%.
|
| Subculturing |
Medium Renewal: Every 2 to 3 days Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml. |
| Cryopreservation |
RPMI 1640 medium with non-essential amino acids, 1 mM sodium pyruvate and 2 mM L-glutamine, 72.5%; fetal bovine serum, 20%; DMSO, 7.5% |
| Isotype |
IgM |
| Name of Depositor |
Centocor, Inc. |
| U.S. Patent Number |
|
| References |
Pollack M, Hunter KW. Monoclonal antibodies reactive with endotoxin core. US Patent 5,057,598 dated Oct 15 1991
|
